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n terminal myc tagged tcf4 truncations  (Addgene inc)


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    Structured Review

    Addgene inc n terminal myc tagged tcf4 truncations
    N Terminal Myc Tagged Tcf4 Truncations, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+myc+tag/pcDNA%2FMyc+TCF4+(Plasmid+%2316512)/pm39789376-590-2-14
    Average 92 stars, based on 20 article reviews
    n terminal myc tagged tcf4 truncations - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Transfection:

    Article Title: Complete Combinatorial Mutational Enumeration of a protein functional site enables sequence-landscape mapping and identifies highly-mutated variants that retain activity
    Article Snippet: .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185). .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185).

    Expressing:

    Article Title: Complete Combinatorial Mutational Enumeration of a protein functional site enables sequence-landscape mapping and identifies highly-mutated variants that retain activity
    Article Snippet: .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185). .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185).

    Plasmid Preparation:

    Article Title: Complete Combinatorial Mutational Enumeration of a protein functional site enables sequence-landscape mapping and identifies highly-mutated variants that retain activity
    Article Snippet: .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185). .. To obtain the ACE2+ transient cell line, HEK293 cells were transiently transfected with a mammalian expression plasmid encoding for human ACE2 with an N-terminal Myc tag (Addgene #141185).

    Article Title: RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer.
    Article Snippet: The pUltra vector (Addgene, 24129)62 was first modified, swapping out the EGFP for a gene encoding puromycin resistance. .. DNA 16 NATURE COMMUNICATIONS | (2022) 13:2791 | https://doi.org/10.1038/s41467-022-30489-z | www.nature.com/naturecommunications sequence encoding the BirA enzyme with an N-terminal Myc tag (Myc-BioID2MC, Addgene, 74223) was then subcloned into the pUltra vector (Addgene, 24129). ..

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis.
    Article Snippet: .. GraphPad Prism 9.4.1. npj Precision Oncology (2023) 20 Published in partnership with The Hormel Institute, University of Minnesota reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis
    Article Snippet: Human colon cancer cell lines (SW48, SW480, SW620, Caco2, HT29, LoVo) and non-transformed colonic epithelial cells (CCD841) were obtained from ATCC and validated as reported , . .. Chemicals, reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Article Title: RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer
    Article Snippet: The pUltra vector (Addgene, 24129) was first modified, swapping out the EGFP for a gene encoding puromycin resistance. .. DNA sequence encoding the BirA enzyme with an N-terminal Myc tag (Myc-BioID2-MC, Addgene, 74223) was then subcloned into the pUltra vector (Addgene, 24129). ..

    Sequencing:

    Article Title: RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer.
    Article Snippet: The pUltra vector (Addgene, 24129)62 was first modified, swapping out the EGFP for a gene encoding puromycin resistance. .. DNA 16 NATURE COMMUNICATIONS | (2022) 13:2791 | https://doi.org/10.1038/s41467-022-30489-z | www.nature.com/naturecommunications sequence encoding the BirA enzyme with an N-terminal Myc tag (Myc-BioID2MC, Addgene, 74223) was then subcloned into the pUltra vector (Addgene, 24129). ..

    Article Title: RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer
    Article Snippet: The pUltra vector (Addgene, 24129) was first modified, swapping out the EGFP for a gene encoding puromycin resistance. .. DNA sequence encoding the BirA enzyme with an N-terminal Myc tag (Myc-BioID2-MC, Addgene, 74223) was then subcloned into the pUltra vector (Addgene, 24129). ..

    Cell Culture:

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis.
    Article Snippet: .. GraphPad Prism 9.4.1. npj Precision Oncology (2023) 20 Published in partnership with The Hormel Institute, University of Minnesota reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis
    Article Snippet: Human colon cancer cell lines (SW48, SW480, SW620, Caco2, HT29, LoVo) and non-transformed colonic epithelial cells (CCD841) were obtained from ATCC and validated as reported , . .. Chemicals, reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    shRNA:

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis.
    Article Snippet: .. GraphPad Prism 9.4.1. npj Precision Oncology (2023) 20 Published in partnership with The Hormel Institute, University of Minnesota reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis
    Article Snippet: Human colon cancer cell lines (SW48, SW480, SW620, Caco2, HT29, LoVo) and non-transformed colonic epithelial cells (CCD841) were obtained from ATCC and validated as reported , . .. Chemicals, reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Over Expression:

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis.
    Article Snippet: .. GraphPad Prism 9.4.1. npj Precision Oncology (2023) 20 Published in partnership with The Hormel Institute, University of Minnesota reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Article Title: Targeting ACE2-BRD4 crosstalk in colorectal cancer and the deregulation of DNA repair and apoptosis
    Article Snippet: Human colon cancer cell lines (SW48, SW480, SW620, Caco2, HT29, LoVo) and non-transformed colonic epithelial cells (CCD841) were obtained from ATCC and validated as reported , . .. Chemicals, reagents, and cell culture media were purchased from Invitrogen, dBET6, JQ1 and MZ1 from MedChemExpress, ACE2 shRNA plasmids (#sc-41400-SH) from Santa Cruz Biotechnology, and ACE2 overexpression plasmid with an N-terminal Myc tag (#141185) from Addgene. ..

    Generated:

    Article Title: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
    Article Snippet: The Nix (Bnip3L pcDNA3.1) and shNix plasmids were a gift from Wafik El-Deiry (Addgene #17467, 17469) [ ]. .. Myc-tagged Nix was generated using Bnip3L pcDNA3.1 as a PCR template and ligating the subsequent amplicon into a pcDNA3 backbone containing an N-terminal Myc tag (EcoRI-XhoI; Addgene #100795). .. Flag-Bcl-2, Flag-Cb5, an Flag-MaoB plasmids were gifted from Clark Distelhorst (Addgene #18003, 18004, and 18005) [ ].

    Polymerase Chain Reaction:

    Article Title: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
    Article Snippet: The Nix (Bnip3L pcDNA3.1) and shNix plasmids were a gift from Wafik El-Deiry (Addgene #17467, 17469) [ ]. .. Myc-tagged Nix was generated using Bnip3L pcDNA3.1 as a PCR template and ligating the subsequent amplicon into a pcDNA3 backbone containing an N-terminal Myc tag (EcoRI-XhoI; Addgene #100795). .. Flag-Bcl-2, Flag-Cb5, an Flag-MaoB plasmids were gifted from Clark Distelhorst (Addgene #18003, 18004, and 18005) [ ].

    Amplification:

    Article Title: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
    Article Snippet: The Nix (Bnip3L pcDNA3.1) and shNix plasmids were a gift from Wafik El-Deiry (Addgene #17467, 17469) [ ]. .. Myc-tagged Nix was generated using Bnip3L pcDNA3.1 as a PCR template and ligating the subsequent amplicon into a pcDNA3 backbone containing an N-terminal Myc tag (EcoRI-XhoI; Addgene #100795). .. Flag-Bcl-2, Flag-Cb5, an Flag-MaoB plasmids were gifted from Clark Distelhorst (Addgene #18003, 18004, and 18005) [ ].



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